Postdoctoral Fellow, Carvalho Lab

The Carvalho Lab at the Pacific Northwest Research Institute (PNRI) in Seattle, WA seeks a proactive and goal-oriented Postdoctoral Fellow who is passionate about genetics and the molecular basis of rare human genetic diseases. The successful candidate will be participating in several collaborative (multi-lab) projects to investigate the structure and molecular mechanisms underlying formation of structural variation in patients and family members with rare genetic diseases. 

Research in the Carvalho Lab aims to investigate the mechanism of formation of de novo variants in the human genome and how they contribute to disease using a wide range of bioinformatic tools and NGS methodologies (please see our publications here. The successful candidate will generate and analyze molecular and computational data from genome (Nanopore, PacBio), methylome and transcriptome from long-read sequencing in addition to investigate ultra-high molecular weight DNA using optical genome mapping. The ideal candidate will develop experiments involving human tissue culture growth and maintenance including lymphoblastoid cell lines and skin fibroblasts, conduct molecular experiments such as quantitative PCR, preparation and analysis of RNA, preparation and quality control of libraries for sequencing, and statistical analysis and summaries of data for publication. The Postdoctoral Fellow will be strongly encouraged to write manuscripts and grants as well as participate in scientific national and international conferences. 

The highly collaborative, interdisciplinary nature of our laboratory provides a strong training environment in the areas of human genetics and genomics, as well as the opportunity to collaborate with world experts in the field of rare diseases and structural variation. The Carvalho Lab and the broader PNRI community actively strive to increase diversity in STEM, translate our discoveries in ways that benefit society, and train the next generation of scientists.

What you will do:

To perform this job successfully, an individual must be able to perform each essential duty satisfactorily. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

  • Design, develop, implement, and execute scientific research under supervision of Principal Investigator.
  • Perform periodical/literature searches in preparation for study design.
  • Maintain substantial knowledge of current scientific and theories.
  • Prepare laboratory equipment and resources.
  • Conduct research, monitor experiments, make observations, and perform hands on observations and activities.
  • Participate in analysis and interpretation of study data.
  • Contribute to scientific literature, grant writing, and conferences in conjunction with Principal Scientist.
  • May develop and implement methodologies, instrumentation, and procedures.
  • May mentor undergraduate and graduate students.

What you bring:

To perform this job successfully, an individual should be able to demonstrate the requirements listed below which are representative of the knowledge, skill, and/or ability required. Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

  • Doctoral degree (PhD, MD, or equivalent) required.
  • Prior experience in a research environment required.
  • Demonstrated knowledge and expertise in genetics and basic molecular technologies required.
  • Verified training and experience with human tissue culture growth and maintenance desired.
  • Proven ability in computational methods and tools for using short and long-read, next-generation sequencing to study the human genome a plus.
  • Demonstrated motivation and ability for applying new technologies to exciting research questions.
  • Demonstrable written and verbal communication skills and the proven ability to thrive in a team environment and work collaboratively as well as independently.
  • Track record of publication in peer-reviewed journals.

PNRI is committed to creating a diverse environment and all qualified candidates are encouraged to apply.

How to apply:

The annual base salary for this position is from $69,264.00 to $73,075.00 and the wage offered will be based on experience, qualifications and the NRSA stipend level.

PNRI requires vaccination for COVID 19 as a condition of employment. Please see for more information.

To apply, please complete and submit your application below by July 31, 2024.

About PNRI

PNRI is an independent, nonprofit, biomedical research institute with a distinguished history of contributing scientific advances to improve health. We believe genetic research holds untapped potential to improve human health. PNRI pursues an unanswered question in the field of genetics: what keeps people healthy in the face of genetic and environmental risk?  At PNRI, our culture encourages originality, risk-taking, and interdisciplinary collaboration. 

PNRI is an equal opportunity employer.  PNRI does not, and will not, discriminate against any applicant or employee on the basis of race, creed, ancestry, color, religion, sex, gender, gender identity/expression, sexual orientation, national origin, citizenship status, mental or physical disability, age, genetic information, veteran status, marital or family status, family or medical care leave, pregnancy or related condition (including childbirth and/or nursing), political affiliation or any other characteristic protected by state or federal law or local ordinance.  We believe diversity, equity, inclusion, and belonging is not only good business, it’s the right thing to do.